Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Transcription factors with Perturb-seq knockdown data for ARHGEF25. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGEF25 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGEF25, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:57,377,252–57,378,039 | 233.7 kb | Distal (>10kb) Multiome | 170 | |
| chr12:57,430,384–57,431,415 | 180.4 kb | Distal (>10kb) Multiome | 879 | |
| chr12:57,454,516–57,456,639 | 155.1 kb | Distal (>10kb) Multiome | 841 | |
| chr12:57,458,743–57,463,318 | 150.1 kb | Distal (>10kb) Multiome | 994 | |
| chr12:57,475,229–57,476,151 | 135.9 kb | Distal (>10kb) Multiome | 194 | |
| chr12:57,487,577–57,488,336 | 123.5 kb | Distal (>10kb) Multiome | 784 | |
| chr12:57,519,488–57,521,440 | 90.8 kb | Distal (>10kb) Multiome | 1029 | |
| chr12:57,521,541–57,523,658 | 88.7 kb | Distal (>10kb) Multiome | 960 | |
| chr12:57,546,771–57,547,419 | 64.2 kb | Distal (>10kb) Multiome | 623 | |
| chr12:57,549,654–57,550,343 | 61.4 kb | Distal (>10kb) Multiome | 366 | |
| chr12:57,583,383–57,583,948 | 27.9 kb | Distal (>10kb) Multiome HiCAR | 353 | |
| chr12:57,590,813–57,591,877 | 20.3 kb | Distal (>10kb) Multiome HiCAR | 738 | |
| chr12:57,604,466–57,605,404 | 6.0 kb | Proximal (<10kb) | 337 | |
| chr12:57,605,566–57,605,777 | 5.7 kb | Proximal (<10kb) | 63 | |
| chr12:57,609,052–57,609,957 | 1.5 kb | Proximal (<10kb) | 83 | |
| chr12:57,610,841–57,611,876 | 22 bp | At TSS Multiome | 558 | |
| chr12:57,614,300–57,614,474 | 2.9 kb | Proximal (<10kb) | 101 | |
| chr12:57,619,368–57,619,897 | 7.9 kb | Proximal (<10kb) | 197 | |
| chr12:57,621,603–57,622,199 | 10.5 kb | Distal (>10kb) Multiome HiCAR | 446 | |
| chr12:57,631,837–57,633,369 | 21.7 kb | Distal (>10kb) Multiome | 706 | |
| chr12:57,693,486–57,694,647 | 82.5 kb | Distal (>10kb) Multiome | 757 | |
| chr12:57,726,079–57,726,879 | 114.8 kb | Distal (>10kb) Multiome | 597 | |
| chr12:57,744,434–57,745,735 | 133.6 kb | Distal (>10kb) Multiome | 822 | |
| chr12:57,751,806–57,753,147 | 141.0 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:57,754,455–57,756,188 | 143.5 kb | Distal (>10kb) Multiome | 616 | |
| chr12:57,765,020–57,765,720 | 153.9 kb | Distal (>10kb) Multiome | 472 | |
| chr12:57,766,452–57,767,514 | 155.6 kb | Distal (>10kb) Multiome | 330 | |
| chr12:57,771,528–57,773,018 | 160.7 kb | Distal (>10kb) Multiome | 1006 | |
| chr12:57,782,579–57,783,204 | 171.3 kb | Distal (>10kb) Multiome | 916 | |
| chr12:57,844,422–57,847,797 | 235.6 kb | Distal (>10kb) Multiome | 1032 | |
| chr12:57,852,281–57,853,469 | 241.4 kb | Distal (>10kb) Multiome | 295 | |
| chr12:57,896,473–57,897,915 | 285.5 kb | Distal (>10kb) Multiome | 419 |
Genomic view of the ARHGEF25 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.