This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ARF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:57,543,921–57,544,886 | 53.0 kb | Distal (>10kb) Multiome | 280 | |
| chr3:57,546,064–57,547,149 | 50.6 kb | Distal (>10kb) Multiome | 113 | |
| chr3:57,555,739–57,556,867 | 41.3 kb | Distal (>10kb) Multiome | 1030 | |
| chr3:57,596,484–57,598,208 | 129 bp | At TSS Multiome | 936 | |
| chr3:57,599,015–57,599,387 | 1.6 kb | Proximal (<10kb) | 122 | |
| chr3:57,692,365–57,693,415 | 95.5 kb | Distal (>10kb) Multiome | 923 | |
| chr3:57,727,873–57,728,338 | 130.7 kb | Distal (>10kb) Multiome | 20 | |
| chr3:57,755,657–57,757,330 | 158.8 kb | Distal (>10kb) Multiome | 930 |
Genomic view of the ARF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.