The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
Transcription factors with Perturb-seq knockdown data for AFF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AFF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AFF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:132,736,904–132,738,172 | 226.1 kb | Distal (>10kb) Multiome | 706 | |
| chr5:132,746,941–132,748,230 | 215.9 kb | Distal (>10kb) Multiome | 369 | |
| chr5:132,776,389–132,777,915 | 186.3 kb | Distal (>10kb) Multiome | 587 | |
| chr5:132,813,194–132,815,033 | 149.1 kb | Distal (>10kb) Multiome | 418 | |
| chr5:132,822,729–132,823,756 | 140.4 kb | Distal (>10kb) Multiome | 258 | |
| chr5:132,825,176–132,826,484 | 137.9 kb | Distal (>10kb) Multiome | 208 | |
| chr5:132,829,647–132,831,156 | 133.3 kb | Distal (>10kb) Multiome | 877 | |
| chr5:132,866,046–132,867,186 | 97.1 kb | Distal (>10kb) Multiome | 955 | |
| chr5:132,962,790–132,962,926 | 707 bp | At TSS | 294 | |
| chr5:132,963,042–132,964,332 | 95 bp | At TSS Multiome | 1068 | |
| chr5:132,969,226–132,969,620 | 5.6 kb | Proximal (<10kb) | 12 | |
| chr5:132,971,808–132,972,399 | 8.2 kb | Proximal (<10kb) | 179 | |
| chr5:133,026,009–133,027,021 | 62.9 kb | Distal (>10kb) Multiome | 754 | |
| chr5:133,051,201–133,052,938 | 88.3 kb | Distal (>10kb) Multiome HiCAR | 1018 |
Genomic view of the AFF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.