Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ACOX3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACOX3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACOX3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:8,157,851–8,159,668 | 269.5 kb | Distal (>10kb) Multiome | 635 | |
| chr4:8,242,133–8,242,751 | 186.0 kb | Distal (>10kb) Multiome HiCAR | 482 | |
| chr4:8,269,411–8,270,406 | 158.6 kb | Distal (>10kb) Multiome HiCAR | 248 | |
| chr4:8,410,587–8,411,261 | 17.6 kb | Distal (>10kb) Multiome | 239 | |
| chr4:8,427,739–8,429,898 | 1.2 kb | Proximal (<10kb) Multiome | 796 | |
| chr4:8,430,194–8,430,421 | 1.7 kb | Proximal (<10kb) | 14 | |
| chr4:8,435,765–8,436,370 | 7.5 kb | Proximal (<10kb) Multiome | 58 | |
| chr4:8,437,244–8,438,165 | 9.3 kb | Proximal (<10kb) Multiome | 79 | |
| chr4:8,440,272–8,441,421 | 12.3 kb | Distal (>10kb) Multiome | 843 | |
| chr4:8,559,567–8,561,304 | 131.5 kb | Distal (>10kb) Multiome HiCAR | 152 | |
| chr4:8,567,886–8,568,408 | 139.8 kb | Distal (>10kb) Multiome | 46 | |
| chr4:8,580,009–8,581,204 | 152.2 kb | Distal (>10kb) Multiome | 591 | |
| chr4:8,592,223–8,593,386 | 164.3 kb | Distal (>10kb) Multiome | 263 | |
| chr4:8,607,476–8,608,065 | 179.3 kb | Distal (>10kb) Multiome | 216 |
Genomic view of the ACOX3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.