Predicted to enable DNA-binding transcription factor activity and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of osteoblast differentiation and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within negative regulation of DNA-templated transcription. Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZNF649. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF649 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF649, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:51,687,813–51,691,477 | 215.8 kb | Distal (>10kb) Multiome | 820 | |
| chr19:51,702,162–51,704,690 | 200.8 kb | Distal (>10kb) Multiome | 758 | |
| chr19:51,904,568–51,905,180 | 37 bp | At TSS Multiome | 662 | |
| chr19:51,907,213–51,907,548 | 2.2 kb | Proximal (<10kb) | 9 | |
| chr19:51,926,700–51,927,523 | 22.1 kb | Distal (>10kb) Multiome | 675 | |
| chr19:51,960,144–51,961,162 | 55.8 kb | Distal (>10kb) Multiome | 242 | |
| chr19:52,007,532–52,008,736 | 103.3 kb | Distal (>10kb) Multiome | 704 | |
| chr19:52,026,275–52,027,092 | 121.7 kb | Distal (>10kb) Multiome | 153 | |
| chr19:52,027,979–52,028,972 | 123.5 kb | Distal (>10kb) Multiome | 837 | |
| chr19:52,048,367–52,049,488 | 143.9 kb | Distal (>10kb) Multiome | 711 | |
| chr19:52,139,087–52,140,222 | 235.0 kb | Distal (>10kb) Multiome | 788 | |
| chr19:52,170,935–52,172,031 | 266.6 kb | Distal (>10kb) Multiome | 404 | |
| chr19:52,189,175–52,190,788 | 285.0 kb | Distal (>10kb) Multiome | 912 |
Genomic view of the ZNF649 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.