The protein encoded by this gene enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ZNF335. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF335 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF335, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:45,764,655–45,765,666 | 207.1 kb | Distal (>10kb) Multiome | 192 | |
| chr20:45,770,709–45,771,657 | 201.0 kb | Distal (>10kb) Multiome | 436 | |
| chr20:45,791,633–45,792,259 | 180.3 kb | Distal (>10kb) Multiome | 882 | |
| chr20:45,812,087–45,813,052 | 159.7 kb | Distal (>10kb) Multiome | 695 | |
| chr20:45,833,493–45,834,622 | 138.3 kb | Distal (>10kb) Multiome | 833 | |
| chr20:45,856,871–45,858,137 | 114.7 kb | Distal (>10kb) Multiome | 884 | |
| chr20:45,880,796–45,881,524 | 91.0 kb | Distal (>10kb) Multiome | 738 | |
| chr20:45,890,127–45,891,800 | 80.8 kb | Distal (>10kb) Multiome | 1095 | |
| chr20:45,910,538–45,912,397 | 59.9 kb | Distal (>10kb) Multiome | 713 | |
| chr20:45,933,591–45,935,780 | 37.1 kb | Distal (>10kb) Multiome | 1195 | |
| chr20:45,971,553–45,972,790 | 30 bp | At TSS Multiome | 1206 | |
| chr20:46,004,647–46,005,315 | 32.8 kb | Distal (>10kb) Multiome | 207 | |
| chr20:46,011,604–46,012,166 | 39.7 kb | Distal (>10kb) Multiome | 239 | |
| chr20:46,013,565–46,014,586 | 41.9 kb | Distal (>10kb) Multiome | 735 | |
| chr20:46,021,209–46,023,166 | 49.8 kb | Distal (>10kb) Multiome | 679 | |
| chr20:46,031,750–46,032,261 | 59.8 kb | Distal (>10kb) Multiome | 227 | |
| chr20:46,058,408–46,058,983 | 86.6 kb | Distal (>10kb) Multiome | 123 | |
| chr20:46,089,092–46,090,355 | 117.7 kb | Distal (>10kb) Multiome | 1047 | |
| chr20:46,091,736–46,092,300 | 120.0 kb | Distal (>10kb) Multiome | 38 | |
| chr20:46,117,897–46,118,755 | 146.1 kb | Distal (>10kb) Multiome | 376 | |
| chr20:46,174,457–46,175,011 | 202.5 kb | Distal (>10kb) Multiome | 277 | |
| chr20:46,268,041–46,268,560 | 296.1 kb | Distal (>10kb) Multiome | 82 |
Genomic view of the ZNF335 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.