Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See ZFP93 (MIM 604749) for additional information on zinc finger proteins.[supplied by OMIM, Jul 2002]
Transcription factors with Perturb-seq knockdown data for ZNF254. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF254 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF254, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:23,074,842–23,075,882 | 958.1 kb | Distal (>10kb) Multiome HiCAR | 399 | |
| chr19:23,116,566–23,117,541 | 916.4 kb | Distal (>10kb) Multiome HiCAR | 257 | |
| chr19:23,249,247–23,251,013 | 783.0 kb | Distal (>10kb) Multiome HiCAR | 457 | |
| chr19:23,254,024–23,254,900 | 778.9 kb | Distal (>10kb) Multiome HiCAR | 62 | |
| chr19:23,273,473–23,274,442 | 759.2 kb | Distal (>10kb) Multiome HiCAR | 565 | |
| chr19:23,277,685–23,278,396 | 755.4 kb | Distal (>10kb) Multiome HiCAR | 71 | |
| chr19:23,394,943–23,396,090 | 637.9 kb | Distal (>10kb) Multiome HiCAR | 575 | |
| chr19:23,415,164–23,416,387 | 617.5 kb | Distal (>10kb) Multiome HiCAR | 222 | |
| chr19:23,758,246–23,759,135 | 274.5 kb | Distal (>10kb) Multiome | 349 | |
| chr19:23,762,593–23,763,893 | 270.3 kb | Distal (>10kb) Multiome | 429 | |
| chr19:24,033,224–24,034,370 | 125 bp | At TSS Multiome | 673 | |
| chr19:24,086,718–24,087,708 | 53.8 kb | Distal (>10kb) Multiome | 390 |
Genomic view of the ZNF254 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.