The encoded protein contains GRF zinc finger (zf-GRF) and transmembrane domains. GRF zinc fingers are found in a number of DNA-binding proteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2017]
Transcription factors with Perturb-seq knockdown data for ZGRF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZGRF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZGRF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:112,361,159–112,361,926 | 275.3 kb | Distal (>10kb) Multiome | 209 | |
| chr4:112,378,884–112,379,732 | 257.5 kb | Distal (>10kb) Multiome | 230 | |
| chr4:112,509,466–112,511,736 | 125.8 kb | Distal (>10kb) Multiome | 299 | |
| chr4:112,514,488–112,516,977 | 120.3 kb | Distal (>10kb) Multiome | 586 | |
| chr4:112,522,782–112,524,704 | 112.8 kb | Distal (>10kb) Multiome | 638 | |
| chr4:112,633,508–112,633,715 | 3.1 kb | Proximal (<10kb) | 65 | |
| chr4:112,636,483–112,637,927 | 315 bp | At TSS Multiome | 891 | |
| chr4:112,641,078–112,641,590 | 4.2 kb | Proximal (<10kb) | 5 | |
| chr4:112,641,720–112,642,218 | 4.9 kb | Proximal (<10kb) | 15 | |
| chr4:112,642,445–112,646,414 | 8.9 kb | Proximal (<10kb) Multiome | 296 | |
| chr4:112,647,946–112,649,404 | 12.0 kb | Distal (>10kb) Multiome | 235 | |
| chr4:112,706,097–112,706,856 | 69.6 kb | Distal (>10kb) Multiome | 313 | |
| chr4:112,746,884–112,747,846 | 110.3 kb | Distal (>10kb) Multiome | 275 | |
| chr4:112,751,041–112,752,361 | 114.8 kb | Distal (>10kb) Multiome | 200 | |
| chr4:112,759,125–112,759,682 | 122.6 kb | Distal (>10kb) Multiome HiCAR | 195 | |
| chr4:112,817,726–112,818,715 | 181.3 kb | Distal (>10kb) Multiome | 463 | |
| chr4:112,854,045–112,854,900 | 217.6 kb | Distal (>10kb) Multiome | 101 | |
| chr4:112,860,814–112,861,553 | 224.3 kb | Distal (>10kb) Multiome | 167 |
Genomic view of the ZGRF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.