Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
Transcription factors with Perturb-seq knockdown data for WRNIP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WRNIP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WRNIP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:1,595,602–1,596,723 | 1169.2 kb | Distal (>10kb) Multiome HiCAR | 325 | |
| chr6:1,596,826–1,597,922 | 1168.0 kb | Distal (>10kb) Multiome HiCAR | 307 | |
| chr6:2,588,949–2,589,819 | 176.1 kb | Distal (>10kb) Multiome | 132 | |
| chr6:2,634,053–2,634,953 | 130.9 kb | Distal (>10kb) Multiome | 729 | |
| chr6:2,764,624–2,766,543 | 143 bp | At TSS Multiome | 1055 | |
| chr6:2,841,174–2,842,113 | 76.3 kb | Distal (>10kb) Multiome | 959 | |
| chr6:2,875,264–2,877,978 | 110.2 kb | Distal (>10kb) Multiome | 812 | |
| chr6:2,902,554–2,903,750 | 137.9 kb | Distal (>10kb) Multiome | 774 | |
| chr6:2,913,547–2,914,632 | 148.4 kb | Distal (>10kb) Multiome | 67 | |
| chr6:2,925,788–2,926,597 | 160.8 kb | Distal (>10kb) Multiome | 244 | |
| chr6:2,927,704–2,929,196 | 162.8 kb | Distal (>10kb) Multiome | 154 | |
| chr6:2,931,916–2,932,749 | 167.0 kb | Distal (>10kb) Multiome | 652 | |
| chr6:2,942,573–2,943,965 | 177.6 kb | Distal (>10kb) Multiome | 222 | |
| chr6:2,952,443–2,953,210 | 187.4 kb | Distal (>10kb) Multiome | 649 | |
| chr6:2,970,836–2,972,016 | 205.9 kb | Distal (>10kb) Multiome | 817 | |
| chr6:2,985,539–2,987,671 | 220.6 kb | Distal (>10kb) Multiome | 402 | |
| chr6:2,987,802–2,990,098 | 224.4 kb | Distal (>10kb) Multiome | 1173 | |
| chr6:2,990,284–2,991,348 | 225.5 kb | Distal (>10kb) Multiome | 509 | |
| chr6:2,999,131–3,000,475 | 234.4 kb | Distal (>10kb) Multiome | 930 | |
| chr6:3,041,670–3,042,232 | 276.5 kb | Distal (>10kb) Multiome | 84 | |
| chr6:3,052,936–3,054,463 | 288.3 kb | Distal (>10kb) Multiome | 864 | |
| chr6:3,063,662–3,064,609 | 298.5 kb | Distal (>10kb) Multiome | 752 |
Genomic view of the WRNIP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.