The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Transcription factors with Perturb-seq knockdown data for WNT5A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WNT5A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WNT5A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:55,481,937–55,483,316 | 4.7 kb | Proximal (<10kb) Multiome | 116 | |
| chr3:55,484,296–55,484,639 | 2.7 kb | Proximal (<10kb) | 44 | |
| chr3:55,484,722–55,485,839 | 1.9 kb | Proximal (<10kb) Multiome | 348 | |
| chr3:55,486,105–55,488,985 | at TSS | At TSS | 509 | |
| chr3:55,489,460–55,489,744 | 2.2 kb | Proximal (<10kb) | 72 | |
| chr3:55,489,853–55,490,182 | 2.5 kb | Proximal (<10kb) | 125 |
Genomic view of the WNT5A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.