The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for WASF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WASF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WASF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:109,944,347–109,945,486 | 234.7 kb | Distal (>10kb) Multiome | 254 | |
| chr6:109,977,901–109,979,311 | 201.4 kb | Distal (>10kb) Multiome | 637 | |
| chr6:110,039,510–110,040,377 | 140.2 kb | Distal (>10kb) Multiome | 368 | |
| chr6:110,173,609–110,173,859 | 5.8 kb | Proximal (<10kb) | 94 | |
| chr6:110,173,972–110,174,206 | 5.5 kb | Proximal (<10kb) | 36 | |
| chr6:110,178,668–110,180,832 | 86 bp | At TSS Multiome | 981 | |
| chr6:110,190,380–110,191,802 | 11.1 kb | Distal (>10kb) Multiome | 120 | |
| chr6:110,357,374–110,358,592 | 178.2 kb | Distal (>10kb) Multiome | 257 |
Genomic view of the WASF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.