The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2I. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2I upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2I, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:1,048,353–1,048,991 | 260.5 kb | Distal (>10kb) Multiome | 220 | |
| chr16:1,088,257–1,088,904 | 220.6 kb | Distal (>10kb) Multiome | 201 | |
| chr16:1,102,896–1,103,532 | 206.1 kb | Distal (>10kb) Multiome | 331 | |
| chr16:1,152,124–1,154,512 | 156.2 kb | Distal (>10kb) Multiome | 341 | |
| chr16:1,300,823–1,301,358 | 8.1 kb | Proximal (<10kb) Multiome | 419 | |
| chr16:1,303,395–1,303,825 | 5.3 kb | Proximal (<10kb) | 252 | |
| chr16:1,308,686–1,309,923 | 37 bp | At TSS Multiome | 953 | |
| chr16:1,331,603–1,332,817 | 22.9 kb | Distal (>10kb) Multiome | 492 | |
| chr16:1,333,312–1,334,056 | 24.5 kb | Distal (>10kb) Multiome | 271 | |
| chr16:1,342,604–1,343,805 | 34.0 kb | Distal (>10kb) Multiome | 560 | |
| chr16:1,351,418–1,352,242 | 42.7 kb | Distal (>10kb) Multiome | 733 | |
| chr16:1,378,844–1,380,439 | 70.6 kb | Distal (>10kb) Multiome | 782 | |
| chr16:1,408,010–1,408,819 | 99.2 kb | Distal (>10kb) Multiome | 546 | |
| chr16:1,413,643–1,415,278 | 105.6 kb | Distal (>10kb) Multiome | 661 | |
| chr16:1,420,269–1,421,402 | 111.7 kb | Distal (>10kb) Multiome | 897 | |
| chr16:1,474,566–1,475,411 | 165.9 kb | Distal (>10kb) Multiome | 915 | |
| chr16:1,492,797–1,493,962 | 184.2 kb | Distal (>10kb) Multiome | 710 |
Genomic view of the UBE2I locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.