The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for UBE2H. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2H upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2H, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:129,775,743–129,776,961 | 176.4 kb | Distal (>10kb) Multiome | 77 | |
| chr7:129,778,637–129,782,868 | 172.6 kb | Distal (>10kb) Multiome | 821 | |
| chr7:129,824,642–129,826,481 | 127.1 kb | Distal (>10kb) Multiome | 809 | |
| chr7:129,829,901–129,830,704 | 122.7 kb | Distal (>10kb) Multiome | 440 | |
| chr7:129,914,750–129,915,367 | 37.8 kb | Distal (>10kb) Multiome | 82 | |
| chr7:129,948,898–129,950,017 | 3.8 kb | Proximal (<10kb) Multiome | 747 | |
| chr7:129,952,295–129,953,395 | 37 bp | At TSS Multiome | 834 | |
| chr7:130,069,977–130,071,012 | 117.5 kb | Distal (>10kb) Multiome | 873 | |
| chr7:130,140,676–130,141,967 | 188.7 kb | Distal (>10kb) Multiome | 371 | |
| chr7:130,204,919–130,205,826 | 252.5 kb | Distal (>10kb) Multiome | 874 |
Genomic view of the UBE2H locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.