The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Transcription factors with Perturb-seq knockdown data for UBE2E1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2E1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2E1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:23,202,243–23,204,183 | 602.9 kb | Distal (>10kb) Multiome HiCAR | 694 | |
| chr3:23,701,633–23,702,476 | 104.0 kb | Distal (>10kb) Multiome | 210 | |
| chr3:23,805,437–23,807,461 | 63 bp | At TSS Multiome | 636 | |
| chr3:23,809,943–23,811,474 | 4.6 kb | Proximal (<10kb) Multiome | 685 | |
| chr3:23,916,365–23,917,803 | 111.1 kb | Distal (>10kb) Multiome | 1082 | |
| chr3:23,938,713–23,939,267 | 133.0 kb | Distal (>10kb) Multiome | 172 | |
| chr3:23,944,552–23,946,922 | 139.1 kb | Distal (>10kb) Multiome | 1112 | |
| chr3:23,953,236–23,954,388 | 147.8 kb | Distal (>10kb) Multiome | 152 |
Genomic view of the UBE2E1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.