Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Transcription factors with Perturb-seq knockdown data for UBA2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBA2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBA2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:34,134,218–34,135,012 | 293.8 kb | Distal (>10kb) Multiome | 833 | |
| chr19:34,171,846–34,173,343 | 256.0 kb | Distal (>10kb) Multiome | 945 | |
| chr19:34,254,036–34,255,321 | 173.8 kb | Distal (>10kb) Multiome | 742 | |
| chr19:34,358,965–34,360,056 | 68.9 kb | Distal (>10kb) Multiome | 865 | |
| chr19:34,364,839–34,365,605 | 63.3 kb | Distal (>10kb) Multiome | 554 | |
| chr19:34,404,022–34,405,020 | 24.0 kb | Distal (>10kb) Multiome | 883 | |
| chr19:34,428,165–34,428,863 | 21 bp | At TSS Multiome | 809 | |
| chr19:34,480,917–34,482,711 | 53.2 kb | Distal (>10kb) Multiome | 520 | |
| chr19:34,501,375–34,501,856 | 73.3 kb | Distal (>10kb) Multiome | 590 | |
| chr19:34,668,047–34,668,472 | 240.1 kb | Distal (>10kb) Multiome | 24 | |
| chr19:34,676,923–34,678,053 | 249.2 kb | Distal (>10kb) Multiome | 728 | |
| chr19:34,733,416–34,734,655 | 305.8 kb | Distal (>10kb) Multiome | 1008 |
Genomic view of the UBA2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.