Predicted to be involved in several processes, including negative regulation of canonical Wnt signaling pathway; negative regulation of osteoblast differentiation; and positive regulation of cell differentiation. Predicted to act upstream of or within regulation of ATP-dependent activity. Predicted to be located in membrane. Predicted to be active in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for TMEM64. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TMEM64 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TMEM64, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:89,983,654–89,985,025 | 661.7 kb | Distal (>10kb) Multiome HiCAR | 1128 | |
| chr8:89,992,539–89,993,861 | 653.1 kb | Distal (>10kb) Multiome HiCAR | 132 | |
| chr8:90,000,653–90,002,462 | 644.8 kb | Distal (>10kb) Multiome HiCAR | 917 | |
| chr8:90,354,517–90,356,009 | 290.9 kb | Distal (>10kb) Multiome | 113 | |
| chr8:90,644,726–90,647,850 | 96 bp | At TSS Multiome | 987 | |
| chr8:90,791,022–90,792,433 | 145.6 kb | Distal (>10kb) Multiome | 362 |
Genomic view of the TMEM64 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.