The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. It is involved in local signaling in the developing and adult nervous system, and it contributes to spinal sensitization and neuropathic pain states. This gene is activated during the stromal response to invasive breast cancer. It may also play a role in inflammatory responses in Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Transcription factors with Perturb-seq knockdown data for THBS4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = THBS4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of THBS4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:79,990,706–79,991,420 | 154 bp | At TSS Multiome | 773 | |
| chr5:80,034,875–80,035,788 | 44.1 kb | Distal (>10kb) Multiome | 189 | |
| chr5:80,093,737–80,094,335 | 102.8 kb | Distal (>10kb) Multiome | 220 | |
| chr5:80,111,066–80,112,197 | 120.2 kb | Distal (>10kb) Multiome | 104 | |
| chr5:80,141,245–80,141,718 | 150.2 kb | Distal (>10kb) Multiome | 179 | |
| chr5:80,180,501–80,181,544 | 189.7 kb | Distal (>10kb) Multiome | 195 | |
| chr5:80,183,069–80,185,467 | 192.1 kb | Distal (>10kb) Multiome | 646 | |
| chr5:80,185,568–80,186,143 | 194.5 kb | Distal (>10kb) Multiome | 70 | |
| chr5:80,204,750–80,205,627 | 213.7 kb | Distal (>10kb) Multiome | 594 | |
| chr5:80,255,259–80,256,736 | 264.8 kb | Distal (>10kb) Multiome | 873 | |
| chr5:80,257,220–80,257,979 | 266.4 kb | Distal (>10kb) Multiome | 496 |
Genomic view of the THBS4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.