This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain that is structurally similar to the kinase domains of testis-specific protein kinase-1 and the LIM motif-containing protein kinases (LIMKs). Its overall structure is most related to the former, indicating that it belongs to the TESK subgroup of the LIMK/TESK family of protein kinases. This gene is predominantly expressed in testis and prostate. The developmental expression pattern of the rat gene in testis suggests an important role for this gene in meitoic stages and/or early stages of spermiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Transcription factors with Perturb-seq knockdown data for TESK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TESK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TESK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:45,010,856–45,013,180 | 478.9 kb | Distal (>10kb) Multiome HiCAR | 909 | |
| chr1:45,205,664–45,207,097 | 284.7 kb | Distal (>10kb) Multiome | 691 | |
| chr1:45,216,712–45,217,234 | 274.1 kb | Distal (>10kb) Multiome | 42 | |
| chr1:45,303,203–45,304,881 | 187.3 kb | Distal (>10kb) Multiome HiCAR | 1034 | |
| chr1:45,315,837–45,316,870 | 175.0 kb | Distal (>10kb) Multiome | 113 | |
| chr1:45,317,165–45,317,643 | 173.7 kb | Distal (>10kb) Multiome | 7 | |
| chr1:45,321,623–45,322,180 | 169.4 kb | Distal (>10kb) Multiome HiCAR | 54 | |
| chr1:45,326,327–45,328,092 | 164.3 kb | Distal (>10kb) Multiome HiCAR | 382 | |
| chr1:45,339,505–45,340,694 | 151.0 kb | Distal (>10kb) Multiome HiCAR | 951 | |
| chr1:45,490,605–45,491,773 | 139 bp | At TSS Multiome | 900 | |
| chr1:45,499,499–45,500,620 | 9.0 kb | Proximal (<10kb) Multiome | 831 | |
| chr1:45,521,163–45,522,604 | 30.8 kb | Distal (>10kb) Multiome | 1155 | |
| chr1:45,550,427–45,551,304 | 59.7 kb | Distal (>10kb) Multiome | 1006 | |
| chr1:45,583,145–45,585,182 | 93.6 kb | Distal (>10kb) Multiome | 997 | |
| chr1:45,622,461–45,623,374 | 131.9 kb | Distal (>10kb) Multiome | 169 | |
| chr1:45,686,052–45,687,740 | 195.9 kb | Distal (>10kb) Multiome | 1015 | |
| chr1:45,687,860–45,688,505 | 197.2 kb | Distal (>10kb) Multiome | 861 | |
| chr1:45,750,186–45,751,093 | 259.6 kb | Distal (>10kb) Multiome | 961 |
Genomic view of the TESK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.