This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]
Transcription factors with Perturb-seq knockdown data for TAGLN. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TAGLN upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TAGLN, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:116,772,462–116,773,416 | 426.4 kb | Distal (>10kb) Multiome HiCAR | 928 | |
| chr11:117,097,570–117,099,243 | 100.9 kb | Distal (>10kb) Multiome | 739 | |
| chr11:117,099,458–117,100,103 | 99.4 kb | Distal (>10kb) Multiome | 62 | |
| chr11:117,143,916–117,144,864 | 55.0 kb | Distal (>10kb) Multiome | 969 | |
| chr11:117,178,198–117,179,718 | 20.6 kb | Distal (>10kb) Multiome | 726 | |
| chr11:117,197,957–117,198,434 | 1.3 kb | Proximal (<10kb) Multiome | 160 | |
| chr11:117,198,962–117,199,797 | 47 bp | At TSS Multiome | 211 | |
| chr11:117,231,703–117,233,431 | 33.2 kb | Distal (>10kb) Multiome | 703 | |
| chr11:117,315,726–117,316,638 | 116.9 kb | Distal (>10kb) Multiome | 619 | |
| chr11:117,327,486–117,328,627 | 128.5 kb | Distal (>10kb) Multiome | 751 | |
| chr11:117,410,817–117,412,004 | 212.0 kb | Distal (>10kb) Multiome | 247 | |
| chr11:117,429,727–117,430,361 | 230.7 kb | Distal (>10kb) Multiome | 126 | |
| chr11:117,443,412–117,443,876 | 244.4 kb | Distal (>10kb) Multiome | 185 |
Genomic view of the TAGLN locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.