This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014]
Transcription factors with Perturb-seq knockdown data for SPRY4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPRY4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPRY4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:142,083,687–142,084,682 | 242.3 kb | Distal (>10kb) Multiome | 113 | |
| chr5:142,108,404–142,109,356 | 217.7 kb | Distal (>10kb) Multiome | 759 | |
| chr5:142,169,261–142,169,978 | 156.9 kb | Distal (>10kb) Multiome HiCAR | 405 | |
| chr5:142,175,265–142,176,514 | 150.6 kb | Distal (>10kb) Multiome HiCAR | 230 | |
| chr5:142,279,944–142,281,216 | 46.1 kb | Distal (>10kb) Multiome | 416 | |
| chr5:142,290,346–142,291,338 | 35.6 kb | Distal (>10kb) Multiome | 377 | |
| chr5:142,295,103–142,296,598 | 30.9 kb | Distal (>10kb) Multiome | 308 | |
| chr5:142,324,006–142,326,913 | 267 bp | At TSS Multiome | 1042 | |
| chr5:142,327,022–142,327,374 | 2.0 kb | Proximal (<10kb) | 65 | |
| chr5:142,327,475–142,327,858 | 2.5 kb | Proximal (<10kb) | 118 | |
| chr5:142,355,728–142,357,672 | 30.6 kb | Distal (>10kb) Multiome | 894 | |
| chr5:142,359,659–142,361,148 | 33.9 kb | Distal (>10kb) Multiome | 721 | |
| chr5:142,362,584–142,363,543 | 36.5 kb | Distal (>10kb) Multiome | 653 | |
| chr5:142,474,915–142,475,538 | 148.8 kb | Distal (>10kb) Multiome | 253 | |
| chr5:142,551,285–142,552,143 | 225.3 kb | Distal (>10kb) Multiome HiCAR | 331 |
Genomic view of the SPRY4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.