This gene encodes a member of the GXGD family of aspartic proteases. The GXGD proteases are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions. This enzyme localizes to endosomes, lysosomes, and the plasma membrane. It cleaves the transmembrane domain of tumor necrosis factor alpha to release the intracellular domain, which triggers cytokine expression in the innate and adaptive immunity pathways. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for SPPL2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPPL2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPPL2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:2,034,637–2,035,967 | 293.6 kb | Distal (>10kb) Multiome | 368 | |
| chr19:2,041,744–2,042,811 | 286.4 kb | Distal (>10kb) Multiome | 586 | |
| chr19:2,049,171–2,052,365 | 277.4 kb | Distal (>10kb) Multiome | 977 | |
| chr19:2,060,703–2,062,560 | 267.1 kb | Distal (>10kb) Multiome | 913 | |
| chr19:2,095,898–2,097,954 | 232.0 kb | Distal (>10kb) Multiome | 945 | |
| chr19:2,150,726–2,152,200 | 176.9 kb | Distal (>10kb) Multiome | 761 | |
| chr19:2,163,561–2,164,587 | 164.8 kb | Distal (>10kb) Multiome | 750 | |
| chr19:2,235,315–2,237,253 | 92.0 kb | Distal (>10kb) Multiome | 1119 | |
| chr19:2,269,007–2,271,589 | 59.3 kb | Distal (>10kb) Multiome | 983 | |
| chr19:2,273,467–2,274,136 | 54.9 kb | Distal (>10kb) Multiome | 825 | |
| chr19:2,327,828–2,329,150 | 62 bp | At TSS Multiome | 759 | |
| chr19:2,424,712–2,426,099 | 96.6 kb | Distal (>10kb) Multiome | 261 | |
| chr19:2,426,254–2,428,729 | 99.1 kb | Distal (>10kb) Multiome | 1035 | |
| chr19:2,456,419–2,457,381 | 128.4 kb | Distal (>10kb) Multiome | 579 | |
| chr19:2,461,902–2,462,483 | 133.4 kb | Distal (>10kb) Multiome | 382 | |
| chr19:2,462,877–2,463,379 | 134.6 kb | Distal (>10kb) Multiome | 764 | |
| chr19:2,474,412–2,477,595 | 147.4 kb | Distal (>10kb) Multiome | 1104 | |
| chr19:2,478,328–2,479,798 | 150.7 kb | Distal (>10kb) Multiome | 488 | |
| chr19:2,489,041–2,489,512 | 160.6 kb | Distal (>10kb) Multiome | 276 | |
| chr19:2,540,631–2,541,164 | 212.3 kb | Distal (>10kb) Multiome | 646 | |
| chr19:2,578,493–2,579,701 | 250.8 kb | Distal (>10kb) Multiome | 763 | |
| chr19:2,581,128–2,581,843 | 252.7 kb | Distal (>10kb) Multiome | 206 | |
| chr19:2,588,132–2,588,925 | 259.8 kb | Distal (>10kb) Multiome | 436 | |
| chr19:2,598,968–2,599,665 | 270.6 kb | Distal (>10kb) Multiome | 683 | |
| chr19:2,602,339–2,602,846 | 273.9 kb | Distal (>10kb) Multiome | 233 | |
| chr19:2,611,021–2,611,484 | 282.6 kb | Distal (>10kb) Multiome | 611 |
Genomic view of the SPPL2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.