The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Transcription factors with Perturb-seq knockdown data for SPP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:87,921,767–87,922,408 | 53.7 kb | Distal (>10kb) Multiome | 58 | |
| chr4:87,973,453–87,973,887 | 1.8 kb | Proximal (<10kb) | 290 | |
| chr4:87,974,968–87,975,292 | 417 bp | At TSS | 100 | |
| chr4:87,975,489–87,975,847 | at TSS | At TSS | 211 | |
| chr4:87,985,565–87,985,918 | 9.9 kb | Proximal (<10kb) | 234 | |
| chr4:88,006,620–88,008,542 | 31.9 kb | Distal (>10kb) Multiome | 543 | |
| chr4:88,158,357–88,159,406 | 183.3 kb | Distal (>10kb) Multiome | 719 | |
| chr4:88,178,032–88,179,178 | 202.8 kb | Distal (>10kb) Multiome | 219 |
Genomic view of the SPP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.