This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for SPOP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPOP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPOP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:49,414,619–49,415,442 | 263.1 kb | Distal (>10kb) Multiome | 829 | |
| chr17:49,486,189–49,486,812 | 191.6 kb | Distal (>10kb) Multiome | 147 | |
| chr17:49,494,780–49,495,773 | 182.9 kb | Distal (>10kb) Multiome | 386 | |
| chr17:49,496,269–49,496,902 | 181.5 kb | Distal (>10kb) Multiome | 440 | |
| chr17:49,497,462–49,497,966 | 180.2 kb | Distal (>10kb) Multiome | 280 | |
| chr17:49,508,179–49,508,962 | 169.5 kb | Distal (>10kb) Multiome | 380 | |
| chr17:49,556,195–49,556,772 | 121.6 kb | Distal (>10kb) Multiome | 636 | |
| chr17:49,567,907–49,568,549 | 110.0 kb | Distal (>10kb) Multiome | 623 | |
| chr17:49,569,780–49,570,501 | 107.9 kb | Distal (>10kb) Multiome HiCAR | 893 | |
| chr17:49,575,427–49,577,397 | 101.5 kb | Distal (>10kb) Multiome HiCAR | 657 | |
| chr17:49,677,242–49,678,492 | 11 bp | At TSS Multiome | 1110 | |
| chr17:49,707,638–49,708,519 | 30.1 kb | Distal (>10kb) Multiome | 927 | |
| chr17:49,763,285–49,764,743 | 86.2 kb | Distal (>10kb) Multiome | 768 | |
| chr17:49,788,330–49,789,442 | 110.4 kb | Distal (>10kb) Multiome | 1068 | |
| chr17:49,851,098–49,852,060 | 173.8 kb | Distal (>10kb) Multiome | 614 | |
| chr17:49,865,367–49,866,210 | 187.7 kb | Distal (>10kb) Multiome | 213 | |
| chr17:49,880,287–49,880,820 | 202.5 kb | Distal (>10kb) Multiome | 493 | |
| chr17:49,910,218–49,910,854 | 232.5 kb | Distal (>10kb) Multiome | 230 | |
| chr17:49,968,328–49,969,629 | 291.0 kb | Distal (>10kb) Multiome | 702 |
Genomic view of the SPOP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.