The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for SMPD4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SMPD4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SMPD4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:129,923,163–129,924,117 | 257.9 kb | Distal (>10kb) Multiome | 183 | |
| chr2:130,144,783–130,145,418 | 36.5 kb | Distal (>10kb) Multiome | 89 | |
| chr2:130,181,118–130,182,559 | 380 bp | At TSS Multiome | 790 | |
| chr2:130,183,864–130,184,117 | 2.3 kb | Proximal (<10kb) | 43 | |
| chr2:130,288,447–130,288,984 | 107.2 kb | Distal (>10kb) Multiome | 232 | |
| chr2:130,327,383–130,328,425 | 146.3 kb | Distal (>10kb) Multiome HiCAR | 215 | |
| chr2:130,336,899–130,337,857 | 155.8 kb | Distal (>10kb) Multiome HiCAR | 861 | |
| chr2:130,341,623–130,343,538 | 161.1 kb | Distal (>10kb) Multiome | 1109 | |
| chr2:130,355,375–130,356,704 | 174.4 kb | Distal (>10kb) Multiome | 816 | |
| chr2:130,371,871–130,373,243 | 191.0 kb | Distal (>10kb) Multiome | 651 | |
| chr2:130,391,075–130,391,923 | 209.9 kb | Distal (>10kb) Multiome | 609 |
Genomic view of the SMPD4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.