Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions, including intracellular pH homeostasis, cell volume regulation, and electroneutral NaCl absorption in epithelia (Xu et al., 2008 [PubMed 18209477]).[supplied by OMIM, Apr 2009]
Transcription factors with Perturb-seq knockdown data for SLC9A8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SLC9A8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SLC9A8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:49,567,583–49,569,009 | 244.7 kb | Distal (>10kb) Multiome | 251 | |
| chr20:49,610,709–49,611,568 | 201.6 kb | Distal (>10kb) Multiome HiCAR | 841 | |
| chr20:49,713,090–49,714,241 | 98.9 kb | Distal (>10kb) Multiome HiCAR | 875 | |
| chr20:49,733,540–49,734,199 | 79.0 kb | Distal (>10kb) Multiome | 15 | |
| chr20:49,762,622–49,763,367 | 49.8 kb | Distal (>10kb) Multiome | 245 | |
| chr20:49,812,498–49,813,345 | 23 bp | At TSS Multiome | 943 | |
| chr20:49,915,234–49,916,043 | 102.8 kb | Distal (>10kb) Multiome | 965 | |
| chr20:49,936,000–49,936,835 | 123.5 kb | Distal (>10kb) Multiome | 878 | |
| chr20:49,979,039–49,979,667 | 166.4 kb | Distal (>10kb) Multiome | 832 | |
| chr20:49,982,091–49,984,748 | 170.0 kb | Distal (>10kb) Multiome | 1116 | |
| chr20:49,997,468–49,998,291 | 184.8 kb | Distal (>10kb) Multiome | 80 | |
| chr20:50,012,403–50,013,184 | 200.0 kb | Distal (>10kb) Multiome | 334 | |
| chr20:50,112,672–50,113,319 | 300.2 kb | Distal (>10kb) Multiome | 839 |
Genomic view of the SLC9A8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.