Nucleotide sugars, which are synthesized in the cytosol or the nucleus, are high-energy donor substrates for glycosyltransferases located in the lumen of the endoplasmic reticulum and Golgi apparatus. Translocation of nucleotide sugars from the cytosol into the lumen compartment is mediated by specific nucleotide sugar transporters, such as SLC35D2 (Suda et al., 2004 [PubMed 15082721]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for SLC35D2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SLC35D2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SLC35D2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:96,217,334–96,218,659 | 165.4 kb | Distal (>10kb) Multiome | 570 | |
| chr9:96,383,184–96,383,893 | 106 bp | At TSS Multiome | 519 | |
| chr9:96,416,915–96,419,539 | 33.6 kb | Distal (>10kb) Multiome | 1128 | |
| chr9:96,449,559–96,451,233 | 66.4 kb | Distal (>10kb) Multiome | 666 | |
| chr9:96,543,037–96,543,508 | 159.6 kb | Distal (>10kb) Multiome | 76 | |
| chr9:96,566,442–96,567,471 | 183.2 kb | Distal (>10kb) Multiome | 819 | |
| chr9:96,618,860–96,620,237 | 236.1 kb | Distal (>10kb) Multiome | 570 | |
| chr9:96,620,809–96,621,291 | 237.4 kb | Distal (>10kb) Multiome | 143 | |
| chr9:96,654,449–96,655,707 | 271.6 kb | Distal (>10kb) Multiome | 706 |
Genomic view of the SLC35D2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.