This gene encodes a PDZ-domain-containing protein that belongs to a family of Shroom-related proteins. This protein may be involved in regulating cell shape in certain tissues. A similar protein in mice is required for proper neurulation. [provided by RefSeq, Jan 2011]
Transcription factors with Perturb-seq knockdown data for SHROOM3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SHROOM3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SHROOM3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:76,305,965–76,307,583 | 279.6 kb | Distal (>10kb) Multiome | 793 | |
| chr4:76,547,041–76,547,302 | 8.3 kb | Proximal (<10kb) | 148 | |
| chr4:76,585,845–76,586,672 | 48 bp | At TSS Multiome | 423 | |
| chr4:76,588,365–76,588,572 | 2.1 kb | Proximal (<10kb) | 50 | |
| chr4:76,590,067–76,590,517 | 4.1 kb | Proximal (<10kb) Multiome | 287 | |
| chr4:76,687,677–76,688,402 | 1.2 kb | Proximal (<10kb) | 136 | |
| chr4:76,689,137–76,689,934 | 103.2 kb | Distal (>10kb) Multiome | 423 | |
| chr4:76,696,194–76,696,677 | 110.1 kb | Distal (>10kb) Multiome | 141 | |
| chr4:76,801,591–76,802,890 | 215.7 kb | Distal (>10kb) Multiome | 200 | |
| chr4:76,867,647–76,869,012 | 282.1 kb | Distal (>10kb) Multiome | 115 | |
| chr4:76,896,697–76,898,709 | 311.9 kb | Distal (>10kb) Multiome | 498 | |
| chr4:76,948,267–76,951,238 | 362.7 kb | Distal (>10kb) Multiome | 968 |
Genomic view of the SHROOM3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.