Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for SEMA4G. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SEMA4G upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SEMA4G, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:100,670,865–100,671,507 | 298.4 kb | Distal (>10kb) Multiome | 364 | |
| chr10:100,680,805–100,681,542 | 288.5 kb | Distal (>10kb) Multiome | 636 | |
| chr10:100,715,555–100,715,963 | 253.6 kb | Distal (>10kb) Multiome | 117 | |
| chr10:100,729,074–100,730,178 | 240.0 kb | Distal (>10kb) Multiome | 304 | |
| chr10:100,739,174–100,740,373 | 229.7 kb | Distal (>10kb) Multiome | 205 | |
| chr10:100,744,447–100,746,357 | 224.1 kb | Distal (>10kb) Multiome | 379 | |
| chr10:100,760,518–100,761,080 | 208.6 kb | Distal (>10kb) Multiome | 105 | |
| chr10:100,787,902–100,788,519 | 181.3 kb | Distal (>10kb) Multiome | 52 | |
| chr10:100,826,838–100,828,148 | 141.6 kb | Distal (>10kb) Multiome | 327 | |
| chr10:100,912,455–100,913,519 | 56.6 kb | Distal (>10kb) Multiome | 906 | |
| chr10:100,968,950–100,970,567 | 107 bp | At TSS Multiome | 681 | |
| chr10:100,987,003–100,987,794 | 17.9 kb | Distal (>10kb) Multiome | 786 | |
| chr10:100,996,424–100,998,696 | 27.5 kb | Distal (>10kb) Multiome | 945 | |
| chr10:100,998,810–101,000,165 | 30.1 kb | Distal (>10kb) Multiome | 845 | |
| chr10:101,018,377–101,019,461 | 49.6 kb | Distal (>10kb) Multiome | 654 | |
| chr10:101,030,588–101,032,521 | 61.7 kb | Distal (>10kb) Multiome | 613 | |
| chr10:101,042,527–101,043,387 | 73.4 kb | Distal (>10kb) Multiome | 655 | |
| chr10:101,048,051–101,048,547 | 78.8 kb | Distal (>10kb) Multiome | 87 | |
| chr10:101,049,966–101,050,753 | 80.9 kb | Distal (>10kb) Multiome | 412 | |
| chr10:101,060,073–101,063,211 | 93.3 kb | Distal (>10kb) Multiome | 1124 | |
| chr10:101,066,181–101,066,983 | 97.0 kb | Distal (>10kb) Multiome | 734 | |
| chr10:101,067,105–101,068,123 | 98.4 kb | Distal (>10kb) Multiome | 643 | |
| chr10:101,095,368–101,096,269 | 126.2 kb | Distal (>10kb) Multiome | 326 | |
| chr10:101,131,094–101,132,387 | 161.9 kb | Distal (>10kb) Multiome | 289 | |
| chr10:101,139,407–101,140,520 | 170.3 kb | Distal (>10kb) Multiome | 158 | |
| chr10:101,161,678–101,162,270 | 192.4 kb | Distal (>10kb) Multiome | 336 | |
| chr10:101,214,601–101,215,378 | 245.5 kb | Distal (>10kb) Multiome | 302 | |
| chr10:101,217,392–101,218,117 | 248.2 kb | Distal (>10kb) Multiome | 203 | |
| chr10:101,236,527–101,237,332 | 267.5 kb | Distal (>10kb) Multiome | 123 |
Genomic view of the SEMA4G locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.