The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]
Transcription factors with Perturb-seq knockdown data for SELENOI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SELENOI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SELENOI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:26,172,352–26,174,365 | 173.1 kb | Distal (>10kb) Multiome HiCAR | 420 | |
| chr2:26,178,689–26,179,345 | 167.0 kb | Distal (>10kb) Multiome | 245 | |
| chr2:26,179,469–26,180,030 | 166.4 kb | Distal (>10kb) Multiome | 122 | |
| chr2:26,184,159–26,185,510 | 161.2 kb | Distal (>10kb) Multiome | 494 | |
| chr2:26,202,471–26,204,141 | 142.3 kb | Distal (>10kb) Multiome | 38 | |
| chr2:26,244,319–26,245,633 | 101.3 kb | Distal (>10kb) Multiome | 928 | |
| chr2:26,339,656–26,339,945 | 6.2 kb | Proximal (<10kb) | 135 | |
| chr2:26,345,352–26,346,859 | 153 bp | At TSS Multiome | 1002 | |
| chr2:26,355,514–26,355,737 | 9.4 kb | Proximal (<10kb) | 324 | |
| chr2:26,401,746–26,402,358 | 55.8 kb | Distal (>10kb) Multiome | 231 | |
| chr2:26,503,215–26,503,885 | 157.4 kb | Distal (>10kb) Multiome | 522 | |
| chr2:26,591,548–26,592,367 | 245.9 kb | Distal (>10kb) Multiome | 47 |
Genomic view of the SELENOI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.