The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Transcription factors with Perturb-seq knockdown data for ROBO2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ROBO2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ROBO2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:75,618,552–75,620,117 | 287.8 kb | Distal (>10kb) Multiome | 134 | |
| chr3:75,906,238–75,907,577 | 83 bp | At TSS Multiome | 182 | |
| chr3:75,915,581–75,915,814 | 8.9 kb | Proximal (<10kb) | 1 | |
| chr3:76,043,182–76,044,358 | 137.2 kb | Distal (>10kb) Multiome | 169 | |
| chr3:77,038,244–77,040,655 | 1133.2 kb | Distal (>10kb) Multiome | 593 | |
| chr3:77,041,380–77,041,575 | 1.3 kb | Proximal (<10kb) | 28 | |
| chr3:78,643,058–78,644,203 | 2737.0 kb | Distal (>10kb) Multiome HiCAR | 145 | |
| chr3:79,023,747–79,025,162 | 3117.6 kb | Distal (>10kb) Multiome HiCAR | 182 |
Genomic view of the ROBO2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.