This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RNF216. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF216 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF216, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:5,486,725–5,487,360 | 294.6 kb | Distal (>10kb) Multiome | 621 | |
| chr7:5,494,650–5,495,099 | 286.9 kb | Distal (>10kb) Multiome | 515 | |
| chr7:5,513,480–5,514,175 | 267.8 kb | Distal (>10kb) Multiome | 815 | |
| chr7:5,524,684–5,527,073 | 255.0 kb | Distal (>10kb) Multiome | 736 | |
| chr7:5,529,088–5,532,313 | 251.7 kb | Distal (>10kb) Multiome | 1081 | |
| chr7:5,554,765–5,555,676 | 226.5 kb | Distal (>10kb) Multiome | 643 | |
| chr7:5,555,801–5,557,053 | 225.1 kb | Distal (>10kb) Multiome | 807 | |
| chr7:5,558,744–5,559,347 | 222.6 kb | Distal (>10kb) Multiome | 525 | |
| chr7:5,562,000–5,564,024 | 219.6 kb | Distal (>10kb) Multiome | 859 | |
| chr7:5,569,367–5,570,943 | 211.4 kb | Distal (>10kb) Multiome | 694 | |
| chr7:5,574,238–5,575,433 | 206.7 kb | Distal (>10kb) Multiome | 447 | |
| chr7:5,591,580–5,596,769 | 188.9 kb | Distal (>10kb) Multiome | 1056 | |
| chr7:5,606,973–5,608,439 | 174.0 kb | Distal (>10kb) Multiome | 322 | |
| chr7:5,779,147–5,779,439 | 2.2 kb | Proximal (<10kb) | 39 | |
| chr7:5,781,073–5,781,919 | 36 bp | At TSS Multiome | 696 | |
| chr7:5,822,727–5,823,386 | 41.4 kb | Distal (>10kb) Multiome | 650 | |
| chr7:6,008,669–6,009,907 | 227.5 kb | Distal (>10kb) Multiome | 1068 | |
| chr7:6,058,614–6,059,749 | 277.5 kb | Distal (>10kb) Multiome | 916 | |
| chr7:6,081,109–6,081,851 | 299.9 kb | Distal (>10kb) Multiome | 904 |
Genomic view of the RNF216 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.