Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in protein polyubiquitination and ubiquitin-dependent protein catabolic process. Located in cytosol and plasma membrane. Biomarker of male infertility. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for RNF114. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF114 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF114, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:49,713,090–49,714,241 | 222.4 kb | Distal (>10kb) Multiome | 875 | |
| chr20:49,733,540–49,734,199 | 202.5 kb | Distal (>10kb) Multiome | 15 | |
| chr20:49,762,622–49,763,367 | 173.3 kb | Distal (>10kb) Multiome | 245 | |
| chr20:49,812,498–49,813,345 | 123.5 kb | Distal (>10kb) Multiome | 943 | |
| chr20:49,915,234–49,916,043 | 20.7 kb | Distal (>10kb) Multiome | 965 | |
| chr20:49,936,000–49,936,835 | 34 bp | At TSS Multiome | 878 | |
| chr20:49,939,454–49,939,847 | 3.1 kb | Proximal (<10kb) | 15 | |
| chr20:49,979,039–49,979,667 | 42.9 kb | Distal (>10kb) Multiome | 832 | |
| chr20:49,982,091–49,984,748 | 46.5 kb | Distal (>10kb) Multiome | 1116 | |
| chr20:49,997,468–49,998,291 | 61.3 kb | Distal (>10kb) Multiome | 80 | |
| chr20:50,012,403–50,013,184 | 76.5 kb | Distal (>10kb) Multiome | 334 | |
| chr20:50,112,672–50,113,319 | 176.7 kb | Distal (>10kb) Multiome | 839 | |
| chr20:50,153,404–50,154,338 | 217.4 kb | Distal (>10kb) Multiome | 1028 | |
| chr20:50,165,812–50,166,377 | 229.7 kb | Distal (>10kb) Multiome | 1037 | |
| chr20:50,171,676–50,172,948 | 236.0 kb | Distal (>10kb) Multiome | 821 | |
| chr20:50,189,686–50,192,514 | 254.2 kb | Distal (>10kb) Multiome | 1125 | |
| chr20:50,194,979–50,195,626 | 259.0 kb | Distal (>10kb) Multiome | 299 |
Genomic view of the RNF114 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.