Enables enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in negative regulation of signal transduction; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein K48-linked ubiquitination. Located in endosome membrane and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for RFFL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RFFL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RFFL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:34,798,552–34,799,335 | 290.4 kb | Distal (>10kb) Multiome | 89 | |
| chr17:34,886,833–34,887,368 | 202.3 kb | Distal (>10kb) Multiome | 205 | |
| chr17:34,961,027–34,961,847 | 127.9 kb | Distal (>10kb) Multiome | 793 | |
| chr17:34,980,134–34,981,317 | 108.7 kb | Distal (>10kb) Multiome | 855 | |
| chr17:35,061,034–35,061,342 | 2.4 kb | Proximal (<10kb) | 28 | |
| chr17:35,063,589–35,064,217 | 25.5 kb | Distal (>10kb) Multiome | 813 | |
| chr17:35,088,426–35,090,139 | 16 bp | At TSS Multiome | 805 | |
| chr17:35,119,302–35,120,200 | 30.5 kb | Distal (>10kb) Multiome | 761 | |
| chr17:35,141,908–35,142,655 | 53.0 kb | Distal (>10kb) Multiome | 873 | |
| chr17:35,242,191–35,243,858 | 153.6 kb | Distal (>10kb) Multiome | 590 | |
| chr17:35,312,777–35,313,483 | 223.7 kb | Distal (>10kb) Multiome | 263 |
Genomic view of the RFFL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.