Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for RCN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RCN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RCN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:30,406,743–30,407,889 | 1683.7 kb | Distal (>10kb) Multiome HiCAR | 242 | |
| chr11:31,797,814–31,799,520 | 292.2 kb | Distal (>10kb) Multiome | 221 | |
| chr11:31,804,516–31,806,498 | 285.0 kb | Distal (>10kb) Multiome | 220 | |
| chr11:31,809,858–31,815,212 | 279.7 kb | Distal (>10kb) Multiome | 719 | |
| chr11:31,816,322–31,818,635 | 273.0 kb | Distal (>10kb) Multiome | 399 | |
| chr11:31,819,537–31,820,941 | 270.7 kb | Distal (>10kb) Multiome | 197 | |
| chr11:31,870,857–31,872,380 | 219.9 kb | Distal (>10kb) Multiome | 359 | |
| chr11:31,926,678–31,927,462 | 164.0 kb | Distal (>10kb) Multiome | 90 | |
| chr11:31,948,279–31,949,502 | 142.3 kb | Distal (>10kb) Multiome | 118 | |
| chr11:32,090,432–32,092,123 | 75 bp | At TSS Multiome | 743 | |
| chr11:32,092,613–32,093,131 | 1.5 kb | Proximal (<10kb) | 88 | |
| chr11:32,333,136–32,334,160 | 242.6 kb | Distal (>10kb) Multiome | 216 | |
| chr11:32,334,700–32,335,307 | 244.0 kb | Distal (>10kb) Multiome | 173 |
Genomic view of the RCN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.