This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Transcription factors with Perturb-seq knockdown data for RBBP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RBBP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RBBP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:32,351,055–32,352,355 | 299.7 kb | Distal (>10kb) Multiome | 944 | |
| chr1:32,361,365–32,362,459 | 289.1 kb | Distal (>10kb) Multiome | 305 | |
| chr1:32,393,928–32,395,356 | 256.6 kb | Distal (>10kb) Multiome | 930 | |
| chr1:32,437,702–32,438,502 | 213.0 kb | Distal (>10kb) Multiome | 85 | |
| chr1:32,439,697–32,440,223 | 211.3 kb | Distal (>10kb) Multiome | 40 | |
| chr1:32,464,697–32,465,552 | 186.2 kb | Distal (>10kb) Multiome | 408 | |
| chr1:32,500,363–32,501,049 | 150.4 kb | Distal (>10kb) Multiome | 540 | |
| chr1:32,520,498–32,520,989 | 130.5 kb | Distal (>10kb) Multiome | 384 | |
| chr1:32,539,285–32,540,081 | 111.5 kb | Distal (>10kb) Multiome | 434 | |
| chr1:32,540,505–32,541,033 | 110.4 kb | Distal (>10kb) Multiome | 172 | |
| chr1:32,611,983–32,612,668 | 38.9 kb | Distal (>10kb) Multiome | 560 | |
| chr1:32,643,807–32,644,727 | 7.0 kb | Proximal (<10kb) Multiome | 70 | |
| chr1:32,650,248–32,651,874 | 170 bp | At TSS Multiome | 1164 | |
| chr1:32,712,054–32,712,688 | 61.2 kb | Distal (>10kb) Multiome | 645 | |
| chr1:32,715,926–32,717,303 | 65.6 kb | Distal (>10kb) Multiome | 551 | |
| chr1:32,736,558–32,737,765 | 85.8 kb | Distal (>10kb) Multiome | 507 | |
| chr1:32,753,606–32,755,507 | 102.8 kb | Distal (>10kb) Multiome | 565 | |
| chr1:32,758,502–32,759,280 | 107.7 kb | Distal (>10kb) Multiome | 144 | |
| chr1:32,765,137–32,765,925 | 114.5 kb | Distal (>10kb) Multiome | 122 | |
| chr1:32,772,938–32,773,451 | 121.9 kb | Distal (>10kb) Multiome | 441 | |
| chr1:32,816,876–32,818,654 | 166.3 kb | Distal (>10kb) Multiome | 1138 | |
| chr1:32,870,570–32,871,148 | 219.6 kb | Distal (>10kb) Multiome | 182 | |
| chr1:32,886,388–32,887,030 | 235.5 kb | Distal (>10kb) Multiome | 917 | |
| chr1:32,892,705–32,893,946 | 241.9 kb | Distal (>10kb) Multiome | 444 | |
| chr1:32,901,143–32,901,775 | 250.3 kb | Distal (>10kb) Multiome | 393 |
Genomic view of the RBBP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.