The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for RASGRP2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RASGRP2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RASGRP2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:64,448,870–64,449,995 | 295.2 kb | Distal (>10kb) Multiome | 490 | |
| chr11:64,630,081–64,630,643 | 114.6 kb | Distal (>10kb) Multiome | 226 | |
| chr11:64,637,888–64,638,531 | 106.6 kb | Distal (>10kb) Multiome | 168 | |
| chr11:64,688,437–64,688,967 | 56.2 kb | Distal (>10kb) Multiome | 581 | |
| chr11:64,710,919–64,711,752 | 33.4 kb | Distal (>10kb) Multiome | 152 | |
| chr11:64,729,775–64,730,315 | 14.8 kb | Distal (>10kb) Multiome | 115 | |
| chr11:64,742,520–64,743,124 | 1.6 kb | Proximal (<10kb) | 501 | |
| chr11:64,743,445–64,743,824 | 2.6 kb | Proximal (<10kb) | 418 | |
| chr11:64,744,623–64,745,186 | 3.7 kb | Proximal (<10kb) | 255 | |
| chr11:64,745,923–64,746,281 | 5.0 kb | Proximal (<10kb) | 202 | |
| chr11:64,759,882–64,760,354 | 15.2 kb | Distal (>10kb) Multiome | 172 | |
| chr11:64,777,493–64,779,271 | 34.0 kb | Distal (>10kb) Multiome | 859 | |
| chr11:64,802,650–64,803,485 | 58.5 kb | Distal (>10kb) Multiome | 522 | |
| chr11:64,809,807–64,811,090 | 65.8 kb | Distal (>10kb) Multiome | 702 | |
| chr11:64,844,075–64,845,264 | 99.9 kb | Distal (>10kb) Multiome | 645 | |
| chr11:64,846,953–64,847,598 | 102.4 kb | Distal (>10kb) Multiome | 509 | |
| chr11:64,877,434–64,879,352 | 133.9 kb | Distal (>10kb) Multiome | 980 | |
| chr11:64,887,785–64,888,374 | 143.2 kb | Distal (>10kb) Multiome | 633 | |
| chr11:64,916,057–64,917,940 | 172.5 kb | Distal (>10kb) Multiome | 873 | |
| chr11:64,924,041–64,924,916 | 179.5 kb | Distal (>10kb) Multiome | 808 | |
| chr11:64,971,835–64,972,454 | 227.3 kb | Distal (>10kb) Multiome | 265 | |
| chr11:65,013,538–65,014,838 | 269.1 kb | Distal (>10kb) Multiome | 980 | |
| chr11:65,027,243–65,027,944 | 282.7 kb | Distal (>10kb) Multiome | 851 | |
| chr11:65,040,595–65,041,884 | 296.2 kb | Distal (>10kb) Multiome | 838 |
Genomic view of the RASGRP2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.