This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for RAP1GAP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAP1GAP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAP1GAP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:21,344,857–21,346,026 | 323.7 kb | Distal (>10kb) Multiome | 850 | |
| chr1:21,436,521–21,437,523 | 232.3 kb | Distal (>10kb) Multiome | 100 | |
| chr1:21,439,762–21,440,452 | 229.2 kb | Distal (>10kb) Multiome | 454 | |
| chr1:21,490,894–21,491,460 | 178.3 kb | Distal (>10kb) Multiome | 154 | |
| chr1:21,491,962–21,492,562 | 177.0 kb | Distal (>10kb) Multiome | 116 | |
| chr1:21,497,823–21,498,256 | 171.3 kb | Distal (>10kb) Multiome | 85 | |
| chr1:21,508,855–21,510,512 | 158.9 kb | Distal (>10kb) Multiome | 399 | |
| chr1:21,607,576–21,608,530 | 61.4 kb | Distal (>10kb) Multiome | 338 | |
| chr1:21,621,983–21,623,132 | 46.8 kb | Distal (>10kb) Multiome | 228 | |
| chr1:21,625,870–21,626,140 | 3.3 kb | Proximal (<10kb) | 47 | |
| chr1:21,632,174–21,632,604 | 9.6 kb | Proximal (<10kb) | 426 | |
| chr1:21,638,784–21,640,464 | 29.6 kb | Distal (>10kb) Multiome | 542 | |
| chr1:21,651,625–21,652,424 | 17.4 kb | Distal (>10kb) Multiome | 367 | |
| chr1:21,659,145–21,659,637 | 10.0 kb | Proximal (<10kb) Multiome | 960 | |
| chr1:21,668,987–21,670,050 | 118 bp | At TSS Multiome | 743 | |
| chr1:21,782,310–21,784,177 | 114.1 kb | Distal (>10kb) Multiome | 1096 | |
| chr1:21,814,299–21,814,932 | 145.2 kb | Distal (>10kb) Multiome | 227 | |
| chr1:21,922,465–21,923,180 | 253.4 kb | Distal (>10kb) Multiome | 263 | |
| chr1:21,936,519–21,938,007 | 268.1 kb | Distal (>10kb) Multiome | 630 |
Genomic view of the RAP1GAP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.