The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
Transcription factors with Perturb-seq knockdown data for RAD52. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAD52 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAD52, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:569,602–571,188 | 379.7 kb | Distal (>10kb) Multiome HiCAR | 800 | |
| chr12:669,635–670,134 | 279.8 kb | Distal (>10kb) Multiome | 33 | |
| chr12:740,453–741,486 | 208.6 kb | Distal (>10kb) Multiome | 114 | |
| chr12:751,121–751,920 | 198.4 kb | Distal (>10kb) Multiome | 860 | |
| chr12:752,026–753,528 | 197.1 kb | Distal (>10kb) Multiome | 747 | |
| chr12:949,091–950,483 | 179 bp | At TSS Multiome | 982 | |
| chr12:990,383–991,780 | 41.5 kb | Distal (>10kb) Multiome | 990 | |
| chr12:1,580,606–1,581,311 | 631.2 kb | Distal (>10kb) Multiome HiCAR | 62 |
Genomic view of the RAD52 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.