This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for RACGAP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RACGAP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RACGAP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:49,741,069–49,742,357 | 284.0 kb | Distal (>10kb) Multiome | 980 | |
| chr12:49,827,122–49,829,288 | 197.0 kb | Distal (>10kb) Multiome | 839 | |
| chr12:49,842,401–49,843,296 | 182.4 kb | Distal (>10kb) Multiome | 806 | |
| chr12:49,866,994–49,867,580 | 158.2 kb | Distal (>10kb) Multiome | 334 | |
| chr12:49,945,408–49,945,886 | 79.7 kb | Distal (>10kb) Multiome | 520 | |
| chr12:49,961,271–49,962,190 | 63.8 kb | Distal (>10kb) Multiome HiCAR | 164 | |
| chr12:49,966,854–49,967,924 | 58.1 kb | Distal (>10kb) Multiome HiCAR | 698 | |
| chr12:50,024,956–50,025,990 | 87 bp | At TSS Multiome | 829 | |
| chr12:50,050,458–50,051,443 | 25.4 kb | Distal (>10kb) Multiome HiCAR | 412 | |
| chr12:50,057,263–50,058,231 | 32.2 kb | Distal (>10kb) Multiome HiCAR | 437 | |
| chr12:50,084,798–50,085,859 | 59.8 kb | Distal (>10kb) Multiome | 715 | |
| chr12:50,110,765–50,112,744 | 86.5 kb | Distal (>10kb) Multiome | 967 | |
| chr12:50,166,692–50,167,931 | 142.0 kb | Distal (>10kb) Multiome | 812 | |
| chr12:50,232,842–50,233,291 | 207.6 kb | Distal (>10kb) Multiome | 105 | |
| chr12:50,283,141–50,284,081 | 258.1 kb | Distal (>10kb) Multiome | 750 |
Genomic view of the RACGAP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.