This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]
Transcription factors with Perturb-seq knockdown data for PYCR2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PYCR2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PYCR2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:225,474,969–225,475,612 | 449.1 kb | Distal (>10kb) Multiome HiCAR | 742 | |
| chr1:225,627,340–225,627,898 | 296.7 kb | Distal (>10kb) Multiome | 254 | |
| chr1:225,652,334–225,654,131 | 271.0 kb | Distal (>10kb) Multiome | 786 | |
| chr1:225,754,870–225,755,548 | 169.2 kb | Distal (>10kb) Multiome | 382 | |
| chr1:225,768,775–225,769,570 | 155.1 kb | Distal (>10kb) Multiome | 313 | |
| chr1:225,771,884–225,772,638 | 151.9 kb | Distal (>10kb) Multiome | 488 | |
| chr1:225,777,167–225,778,412 | 146.5 kb | Distal (>10kb) Multiome | 942 | |
| chr1:225,809,711–225,811,548 | 114.1 kb | Distal (>10kb) Multiome | 654 | |
| chr1:225,845,424–225,846,556 | 78.3 kb | Distal (>10kb) Multiome | 416 | |
| chr1:225,881,255–225,884,173 | 41.9 kb | Distal (>10kb) Multiome | 879 | |
| chr1:225,888,552–225,889,596 | 35.1 kb | Distal (>10kb) Multiome | 185 | |
| chr1:225,898,735–225,899,922 | 24.8 kb | Distal (>10kb) Multiome | 335 | |
| chr1:225,903,782–225,904,454 | 20.3 kb | Distal (>10kb) Multiome | 40 | |
| chr1:225,908,825–225,909,327 | 15.3 kb | Distal (>10kb) Multiome | 101 | |
| chr1:225,910,978–225,911,799 | 12.9 kb | Distal (>10kb) Multiome | 344 | |
| chr1:225,923,776–225,924,773 | 66 bp | At TSS Multiome | 960 | |
| chr1:225,931,396–225,931,784 | 7.1 kb | Proximal (<10kb) | 172 | |
| chr1:225,939,444–225,939,998 | 15.4 kb | Distal (>10kb) Multiome | 62 | |
| chr1:225,945,518–225,946,038 | 21.5 kb | Distal (>10kb) Multiome | 172 | |
| chr1:225,948,616–225,949,435 | 24.8 kb | Distal (>10kb) Multiome | 175 | |
| chr1:225,957,776–225,958,797 | 34.1 kb | Distal (>10kb) Multiome | 175 | |
| chr1:225,999,043–226,000,159 | 75.2 kb | Distal (>10kb) Multiome | 969 | |
| chr1:226,061,587–226,063,333 | 138.2 kb | Distal (>10kb) Multiome | 976 | |
| chr1:226,082,913–226,084,135 | 159.3 kb | Distal (>10kb) Multiome | 852 | |
| chr1:226,109,567–226,111,083 | 185.8 kb | Distal (>10kb) Multiome | 768 | |
| chr1:226,121,192–226,122,943 | 197.6 kb | Distal (>10kb) Multiome | 840 | |
| chr1:226,125,045–226,125,680 | 201.1 kb | Distal (>10kb) Multiome | 373 | |
| chr1:226,186,167–226,187,132 | 262.5 kb | Distal (>10kb) Multiome | 939 | |
| chr1:226,210,358–226,211,556 | 286.6 kb | Distal (>10kb) Multiome | 262 | |
| chr1:226,214,653–226,215,688 | 291.1 kb | Distal (>10kb) Multiome | 145 | |
| chr1:226,222,744–226,224,297 | 299.1 kb | Distal (>10kb) Multiome | 635 |
Genomic view of the PYCR2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.