Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]
Transcription factors with Perturb-seq knockdown data for PUS10. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PUS10 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PUS10, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:60,735,617–60,736,154 | 282.4 kb | Distal (>10kb) Multiome | 172 | |
| chr2:60,756,026–60,757,043 | 262.0 kb | Distal (>10kb) Multiome | 703 | |
| chr2:60,853,117–60,853,733 | 164.9 kb | Distal (>10kb) Multiome | 48 | |
| chr2:60,880,748–60,882,118 | 136.8 kb | Distal (>10kb) Multiome | 991 | |
| chr2:61,016,845–61,018,378 | 827 bp | At TSS Multiome | 1133 | |
| chr2:61,065,384–61,066,693 | 47.6 kb | Distal (>10kb) Multiome | 856 | |
| chr2:61,144,728–61,145,208 | 126.7 kb | Distal (>10kb) Multiome | 512 | |
| chr2:61,157,943–61,158,813 | 140.3 kb | Distal (>10kb) Multiome | 49 | |
| chr2:61,165,843–61,166,373 | 147.9 kb | Distal (>10kb) Multiome | 88 | |
| chr2:61,176,826–61,178,378 | 159.1 kb | Distal (>10kb) Multiome | 1042 |
Genomic view of the PUS10 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.