This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PUM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PUM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PUM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:30,782,240–30,782,828 | 283.1 kb | Distal (>10kb) Multiome | 279 | |
| chr1:30,801,625–30,802,230 | 263.8 kb | Distal (>10kb) Multiome | 372 | |
| chr1:30,824,074–30,824,916 | 241.2 kb | Distal (>10kb) Multiome | 678 | |
| chr1:30,837,666–30,838,184 | 227.8 kb | Distal (>10kb) Multiome | 67 | |
| chr1:30,908,653–30,909,448 | 156.9 kb | Distal (>10kb) Multiome | 272 | |
| chr1:31,062,544–31,063,038 | 2.7 kb | Proximal (<10kb) | 174 | |
| chr1:31,065,191–31,066,200 | 89 bp | At TSS Multiome | 984 | |
| chr1:31,072,313–31,072,760 | 7.0 kb | Proximal (<10kb) Multiome | 160 | |
| chr1:31,072,896–31,073,327 | 7.2 kb | Proximal (<10kb) | 72 | |
| chr1:31,154,613–31,155,769 | 89.4 kb | Distal (>10kb) Multiome | 807 | |
| chr1:31,162,718–31,163,222 | 97.2 kb | Distal (>10kb) Multiome | 461 | |
| chr1:31,182,154–31,182,689 | 116.7 kb | Distal (>10kb) Multiome | 644 | |
| chr1:31,239,028–31,240,122 | 173.9 kb | Distal (>10kb) Multiome | 323 | |
| chr1:31,296,477–31,297,658 | 231.2 kb | Distal (>10kb) Multiome | 749 |
Genomic view of the PUM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.