The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PSMF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:833,367–834,334 | 285.0 kb | Distal (>10kb) Multiome | 839 | |
| chr20:841,821–842,567 | 276.2 kb | Distal (>10kb) Multiome | 248 | |
| chr20:844,437–845,353 | 274.0 kb | Distal (>10kb) Multiome | 299 | |
| chr20:884,981–885,530 | 233.4 kb | Distal (>10kb) Multiome HiCAR | 183 | |
| chr20:955,007–955,547 | 163.3 kb | Distal (>10kb) Multiome HiCAR | 24 | |
| chr20:1,001,978–1,002,815 | 116.2 kb | Distal (>10kb) Multiome | 124 | |
| chr20:1,061,388–1,061,918 | 57.0 kb | Distal (>10kb) Multiome | 105 | |
| chr20:1,108,503–1,108,724 | 9.9 kb | Proximal (<10kb) | 210 | |
| chr20:1,114,166–1,114,356 | 4.3 kb | Proximal (<10kb) | 95 | |
| chr20:1,118,327–1,119,071 | 41 bp | At TSS Multiome | 1066 | |
| chr20:1,184,818–1,186,197 | 67.2 kb | Distal (>10kb) Multiome | 557 | |
| chr20:1,225,365–1,226,806 | 107.2 kb | Distal (>10kb) Multiome | 616 | |
| chr20:1,266,029–1,266,958 | 147.8 kb | Distal (>10kb) Multiome | 499 | |
| chr20:1,300,911–1,301,500 | 182.7 kb | Distal (>10kb) Multiome | 171 | |
| chr20:1,313,163–1,313,790 | 194.9 kb | Distal (>10kb) Multiome | 428 | |
| chr20:1,325,008–1,326,107 | 206.8 kb | Distal (>10kb) Multiome | 901 | |
| chr20:1,392,628–1,393,457 | 274.5 kb | Distal (>10kb) Multiome | 799 |
Genomic view of the PSMF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.