The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PSMC3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMC3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMC3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:47,160,949–47,161,864 | 264.9 kb | Distal (>10kb) Multiome | 322 | |
| chr11:47,176,472–47,177,856 | 249.4 kb | Distal (>10kb) Multiome | 857 | |
| chr11:47,184,866–47,187,095 | 239.9 kb | Distal (>10kb) Multiome | 849 | |
| chr11:47,214,114–47,215,529 | 211.6 kb | Distal (>10kb) Multiome | 785 | |
| chr11:47,248,482–47,249,399 | 177.5 kb | Distal (>10kb) Multiome | 1059 | |
| chr11:47,257,299–47,258,204 | 168.6 kb | Distal (>10kb) Multiome | 519 | |
| chr11:47,259,310–47,259,960 | 166.8 kb | Distal (>10kb) Multiome | 270 | |
| chr11:47,268,988–47,270,613 | 156.6 kb | Distal (>10kb) Multiome | 884 | |
| chr11:47,354,896–47,355,720 | 71.1 kb | Distal (>10kb) Multiome | 670 | |
| chr11:47,399,791–47,400,763 | 26.1 kb | Distal (>10kb) Multiome | 450 | |
| chr11:47,407,671–47,409,059 | 18.0 kb | Distal (>10kb) Multiome | 986 | |
| chr11:47,425,998–47,427,263 | 82 bp | At TSS Multiome | 879 | |
| chr11:47,508,005–47,509,036 | 82.1 kb | Distal (>10kb) Multiome | 73 | |
| chr11:47,552,255–47,553,700 | 126.8 kb | Distal (>10kb) Multiome | 834 | |
| chr11:47,564,988–47,566,330 | 139.2 kb | Distal (>10kb) Multiome | 842 | |
| chr11:47,578,427–47,579,510 | 152.5 kb | Distal (>10kb) Multiome | 1080 | |
| chr11:47,589,692–47,590,847 | 163.9 kb | Distal (>10kb) Multiome | 161 | |
| chr11:47,594,122–47,594,733 | 167.9 kb | Distal (>10kb) Multiome | 153 | |
| chr11:47,598,993–47,599,885 | 172.9 kb | Distal (>10kb) Multiome | 67 | |
| chr11:47,642,000–47,643,006 | 216.1 kb | Distal (>10kb) Multiome | 973 | |
| chr11:47,714,892–47,715,563 | 288.9 kb | Distal (>10kb) Multiome | 607 |
Genomic view of the PSMC3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.