Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRPF8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRPF8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRPF8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:1,399,339–1,400,718 | 284.6 kb | Distal (>10kb) Multiome | 879 | |
| chr17:1,455,335–1,456,916 | 228.5 kb | Distal (>10kb) Multiome | 879 | |
| chr17:1,484,963–1,486,119 | 199.5 kb | Distal (>10kb) Multiome | 562 | |
| chr17:1,487,906–1,488,733 | 196.6 kb | Distal (>10kb) Multiome | 597 | |
| chr17:1,490,717–1,492,156 | 193.4 kb | Distal (>10kb) Multiome | 675 | |
| chr17:1,515,722–1,517,330 | 168.1 kb | Distal (>10kb) Multiome | 796 | |
| chr17:1,562,314–1,563,047 | 122.0 kb | Distal (>10kb) Multiome | 685 | |
| chr17:1,589,843–1,590,457 | 94.7 kb | Distal (>10kb) Multiome | 538 | |
| chr17:1,627,811–1,629,617 | 56.1 kb | Distal (>10kb) Multiome | 753 | |
| chr17:1,642,697–1,644,482 | 41.5 kb | Distal (>10kb) Multiome | 467 | |
| chr17:1,648,163–1,650,244 | 35.7 kb | Distal (>10kb) Multiome | 932 | |
| chr17:1,684,343–1,685,342 | 64 bp | At TSS Multiome | 728 | |
| chr17:1,710,017–1,710,765 | 25.6 kb | Distal (>10kb) Multiome | 500 | |
| chr17:1,713,886–1,717,908 | 32.6 kb | Distal (>10kb) Multiome | 1042 | |
| chr17:1,724,373–1,725,206 | 39.9 kb | Distal (>10kb) Multiome | 720 | |
| chr17:1,829,368–1,830,287 | 145.1 kb | Distal (>10kb) Multiome | 660 | |
| chr17:1,908,573–1,908,999 | 223.8 kb | Distal (>10kb) Multiome | 239 | |
| chr17:1,931,964–1,932,566 | 247.4 kb | Distal (>10kb) Multiome | 198 | |
| chr17:1,960,870–1,961,658 | 276.5 kb | Distal (>10kb) Multiome | 192 |
Genomic view of the PRPF8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.