Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRPF4B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRPF4B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRPF4B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:3,750,069–3,752,509 | 269.2 kb | Distal (>10kb) Multiome | 815 | |
| chr6:3,848,709–3,849,895 | 172.0 kb | Distal (>10kb) Multiome | 495 | |
| chr6:4,018,202–4,019,132 | 2.6 kb | Proximal (<10kb) Multiome | 1077 | |
| chr6:4,020,917–4,022,040 | 86 bp | At TSS Multiome | 909 | |
| chr6:4,078,571–4,079,606 | 57.8 kb | Distal (>10kb) Multiome | 164 | |
| chr6:4,134,917–4,136,384 | 114.5 kb | Distal (>10kb) Multiome | 976 |
Genomic view of the PRPF4B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.