Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRKCZ. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKCZ upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKCZ, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:1,777,637–1,780,173 | 271.7 kb | Distal (>10kb) Multiome | 970 | |
| chr1:1,782,008–1,783,229 | 268.2 kb | Distal (>10kb) Multiome | 590 | |
| chr1:1,883,184–1,883,831 | 166.9 kb | Distal (>10kb) Multiome | 303 | |
| chr1:1,890,319–1,891,952 | 159.2 kb | Distal (>10kb) Multiome | 748 | |
| chr1:1,908,308–1,910,312 | 141.3 kb | Distal (>10kb) Multiome | 801 | |
| chr1:1,918,258–1,920,500 | 131.0 kb | Distal (>10kb) Multiome | 820 | |
| chr1:1,959,901–1,960,517 | 90.3 kb | Distal (>10kb) Multiome HiCAR | 594 | |
| chr1:2,019,137–2,019,982 | 31.0 kb | Distal (>10kb) Multiome | 288 | |
| chr1:2,044,569–2,045,304 | 5.5 kb | Proximal (<10kb) Multiome | 403 | |
| chr1:2,046,521–2,046,771 | 3.7 kb | Proximal (<10kb) | 352 | |
| chr1:2,047,086–2,047,770 | 2.7 kb | Proximal (<10kb) | 290 | |
| chr1:2,049,277–2,050,992 | 198 bp | At TSS Multiome | 732 | |
| chr1:2,132,340–2,134,100 | 83.0 kb | Distal (>10kb) Multiome | 508 | |
| chr1:2,189,382–2,190,314 | 139.2 kb | Distal (>10kb) Multiome | 719 | |
| chr1:2,194,502–2,195,788 | 144.5 kb | Distal (>10kb) Multiome | 789 | |
| chr1:2,199,148–2,199,756 | 149.0 kb | Distal (>10kb) Multiome | 445 | |
| chr1:2,204,191–2,206,396 | 154.7 kb | Distal (>10kb) Multiome | 597 | |
| chr1:2,212,255–2,213,305 | 162.2 kb | Distal (>10kb) Multiome | 601 | |
| chr1:2,226,557–2,228,163 | 177.4 kb | Distal (>10kb) Multiome | 675 | |
| chr1:2,248,086–2,248,655 | 197.9 kb | Distal (>10kb) Multiome | 296 | |
| chr1:2,279,142–2,279,636 | 228.9 kb | Distal (>10kb) Multiome | 172 | |
| chr1:2,311,328–2,311,918 | 261.2 kb | Distal (>10kb) Multiome | 366 | |
| chr1:2,314,340–2,315,728 | 264.6 kb | Distal (>10kb) Multiome | 557 | |
| chr1:2,391,076–2,392,400 | 341.2 kb | Distal (>10kb) Multiome | 820 | |
| chr1:2,411,953–2,414,836 | 362.2 kb | Distal (>10kb) Multiome | 918 |
Genomic view of the PRKCZ locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.