PRKCI
protein kinase C iota | PKCI, DXS1179E

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

Member of: DE-4 DE-4.19 Developmental clusters: GC1
Biological processes 70 terms
ATP binding (GO:0005524)ATP binding (GO:0005524)Golgi membrane (GO:0000139)Golgi vesicle budding (GO:0048194)Schaffer collateral - CA1 synapse (GO:0098685)Schmidt-Lanterman incisure (GO:0043220)apical part of cell (GO:0045177)apical plasma membrane (GO:0016324)bicellular tight junction (GO:0005923)brush border (GO:0005903)cell leading edge (GO:0031252)cell migration (GO:0016477)cell-cell junction organization (GO:0045216)cellular response to chemical stress (GO:0062197)cellular response to insulin stimulus (GO:0032869)cellular response to insulin stimulus (GO:0032869)cellular response to insulin stimulus (GO:0032869)cilium (GO:0005929)cytoplasm (GO:0005737)cytoskeleton organization (GO:0007010)cytosol (GO:0005829)cytosol (GO:0005829)diacylglycerol-dependent serine/threonine kinase activity (GO:0004697)diacylglycerol-dependent, calcium-independent serine/threonine kinase activity (GO:0004699)diacylglycerol-dependent, calcium-independent serine/threonine kinase activity (GO:0004699)endosome (GO:0005768)establishment of cell polarity (GO:0030010)establishment or maintenance of epithelial cell apical/basal polarity (GO:0045197)establishment or maintenance of epithelial cell apical/basal polarity (GO:0045197)extracellular exosome (GO:0070062)glutamatergic synapse (GO:0098978)intracellular signal transduction (GO:0035556)membrane (GO:0016020)membrane organization (GO:0061024)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of glial cell apoptotic process (GO:0034351)negative regulation of neuron apoptotic process (GO:0043524)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)phospholipid binding (GO:0005543)plasma membrane (GO:0005886)positive regulation of D-glucose import across plasma membrane (GO:0046326)positive regulation of D-glucose import across plasma membrane (GO:0046326)positive regulation of Notch signaling pathway (GO:0045747)positive regulation of endothelial cell apoptotic process (GO:2000353)positive regulation of glial cell proliferation (GO:0060252)positive regulation of neuron projection development (GO:0010976)positive regulation of neuron projection development (GO:0010976)positive regulation of protein localization to plasma membrane (GO:1903078)positive regulation of protein localization to plasma membrane (GO:1903078)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein kinase activity (GO:0004672)protein localization to plasma membrane (GO:0072659)protein phosphorylation (GO:0006468)protein serine kinase activity (GO:0106310)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein serine/threonine kinase activity (GO:0004674)protein targeting to membrane (GO:0006612)regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072)response to interleukin-1 (GO:0070555)response to peptide hormone (GO:0043434)secretion (GO:0046903)tight junction (GO:0070160)vesicle-mediated transport (GO:0016192)
Expression (TPM)
PRKCI — as a Regulated Gene

TFs regulating PRKCI 0 TFs

Transcription factors with Perturb-seq knockdown data for PRKCI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKCI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PRKCI

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKCI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr3:169,966,015–169,967,550 255.7 kb Distal (>10kb) Multiome 1017
chr3:170,037,354–170,039,248 184.4 kb Distal (>10kb) Multiome 804
chr3:170,180,833–170,182,100 40.7 kb Distal (>10kb) Multiome 813
chr3:170,221,926–170,223,285 65 bp At TSS Multiome 847
chr3:170,356,326–170,358,653 134.8 kb Distal (>10kb) Multiome 971
chr3:170,418,375–170,421,188 196.3 kb Distal (>10kb) Multiome 413
chr3:170,425,589–170,426,593 203.7 kb Distal (>10kb) Multiome 432
chr3:170,476,158–170,477,136 254.2 kb Distal (>10kb) Multiome 116

Genome Browser

Genomic view of the PRKCI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr3:169,956,015 – 170,487,136
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq