Transcription factors with Perturb-seq knockdown data for PRDM16-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRDM16-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRDM16-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:3,063,064–3,063,280 | 505 bp | At TSS | 43 | |
| chr1:3,063,543–3,063,992 | at TSS | At TSS | 107 | |
| chr1:3,067,788–3,068,493 | 4.0 kb | Proximal (<10kb) | 86 | |
| chr1:3,069,590–3,070,991 | 5.8 kb | Proximal (<10kb) | 387 |
Genomic view of the PRDM16-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.