PPP3CA
protein phosphatase 3 catalytic subunit alpha | CNA1, PPP2B, CALN, CALNA

Enables several functions, including ATPase binding activity; calmodulin binding activity; and calmodulin-dependent protein phosphatase activity. Involved in several processes, including calcineurin-NFAT signaling cascade; negative regulation of angiotensin-activated signaling pathway; and peptidyl-serine dephosphorylation. Located in cytoplasm; cytoplasmic side of plasma membrane; and dendritic spine. Part of calcineurin complex. Implicated in developmental and epileptic encephalopathy 91. Biomarker of cholangiocarcinoma; focal segmental glomerulosclerosis; and schizophrenia. [provided by Alliance of Genome Resources, Jul 2025]

Member of: DE-3 DE-3.6 Developmental clusters: GC4
Biological processes 72 terms
ATPase binding (GO:0051117)T cell activation (GO:0042110)Z disc (GO:0030018)calcineurin complex (GO:0005955)calcineurin complex (GO:0005955)calcineurin complex (GO:0005955)calcineurin-NFAT signaling cascade (GO:0033173)calcineurin-NFAT signaling cascade (GO:0033173)calcineurin-NFAT signaling cascade (GO:0033173)calcineurin-NFAT signaling cascade (GO:0033173)calcineurin-mediated signaling (GO:0097720)calcineurin-mediated signaling (GO:0097720)calcium ion binding (GO:0005509)calmodulin binding (GO:0005516)calmodulin binding (GO:0005516)calmodulin binding (GO:0005516)calmodulin binding (GO:0005516)calmodulin binding (GO:0005516)calmodulin-dependent protein phosphatase activity (GO:0033192)calmodulin-dependent protein phosphatase activity (GO:0033192)calmodulin-dependent protein phosphatase activity (GO:0033192)calmodulin-dependent protein phosphatase activity (GO:0033192)calmodulin-dependent protein phosphatase activity (GO:0033192)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasmic side of plasma membrane (GO:0009898)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)dendritic spine (GO:0043197)dendritic spine (GO:0043197)dephosphorylation (GO:0016311)enzyme binding (GO:0019899)epidermis development (GO:0008544)extrinsic component of plasma membrane (GO:0019897)hydrolase activity (GO:0016787)keratinocyte differentiation (GO:0030216)modulation of chemical synaptic transmission (GO:0050804)negative regulation of angiotensin-activated signaling pathway (GO:0110062)negative regulation of calcium ion import across plasma membrane (GO:1905949)negative regulation of signaling (GO:0023057)nucleoplasm (GO:0005654)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of activated T cell proliferation (GO:0042104)positive regulation of calcineurin-NFAT signaling cascade (GO:0070886)positive regulation of calcium ion import across plasma membrane (GO:1905665)positive regulation of calcium ion-dependent exocytosis of neurotransmitter (GO:1903235)positive regulation of cell adhesion (GO:0045785)positive regulation of cell migration (GO:0030335)positive regulation of glomerulus development (GO:0090193)positive regulation of osteoblast differentiation (GO:0045669)positive regulation of osteoclast differentiation (GO:0045672)positive regulation of saliva secretion (GO:0046878)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein dephosphorylation (GO:0006470)protein dephosphorylation (GO:0006470)protein dimerization activity (GO:0046983)protein serine/threonine phosphatase activity (GO:0004722)protein serine/threonine phosphatase activity (GO:0004722)protein serine/threonine phosphatase activity (GO:0004722)protein serine/threonine phosphatase activity (GO:0004722)regulation of cell proliferation involved in kidney morphogenesis (GO:0061006)renal filtration (GO:0097205)response to calcium ion (GO:0051592)sarcolemma (GO:0042383)skeletal muscle fiber development (GO:0048741)skeletal muscle tissue regeneration (GO:0043403)wound healing (GO:0042060)
Expression (TPM)
PPP3CA — as a Regulated Gene

TFs regulating PPP3CA 0 TFs

Transcription factors with Perturb-seq knockdown data for PPP3CA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP3CA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PPP3CA

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP3CA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr4:101,114,829–101,115,431 232.3 kb Distal (>10kb) Multiome 336
chr4:101,152,402–101,153,947 194.6 kb Distal (>10kb) Multiome 157
chr4:101,282,161–101,282,877 64.9 kb Distal (>10kb) Multiome 222
chr4:101,338,485–101,338,734 8.8 kb Proximal (<10kb) 35
chr4:101,345,356–101,348,852 221 bp At TSS Multiome 986
chr4:101,464,187–101,465,046 117.3 kb Distal (>10kb) Multiome 70

Genome Browser

Genomic view of the PPP3CA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr4:101,104,829 – 101,475,046
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq